RP-HPLC法测定芎菊上清丸中绿原酸的含量

Determination of content of Chlorogenic acid of Xiongju shangqing pills by reversed-phase HPLC

目的建立以RP-HPLC法测定芎菊上清丸中绿原酸含量的方法。方法色谱柱为SHIM-PACKVP-ODS(150mm×4.6mm,5μm),流动相为乙腈-0.4%磷酸水溶液(12∶88),流速为1.0ml/min,检测波长为327nm,柱温为30℃。结果绿原酸进样量在0.0090～0.1125μg范围内与峰面积积分值呈良好线性关系(r=0.9993);平均回收率为99.26%,RSD=0.88%(n=6)。结论本方法简便、快速、准确,可用于芎菊上清丸的质量控制。

Objective To estabish a Reversed-Phase HPLC method for the determination of Chlorogenic acid content in Xiongju shangqing pills.Methods The determination was made using SHIM-PACK VP-ODS column(150 mm×4.6 mm,5 μm)with column temperature at 30℃.The mobile phase was acetonitril-0.4% phosphoric acid(12∶88)with flow rate at 1.0 ml/min.The detective wavelength was set at 327 nm.Results The linear range of Chlorogenic acid was 0.009 0～0.112 5 μg(r=0.999 3).The mean recovery was 99.26%(RSD=1.86%,n=6).Conclusion The...