TTV在非甲～庚型肝炎患者中的检测及序列分析

Detection of TTV infection and sequence analysis in patients with nonA - G hepatitis

目的研究TTV在非甲-庚型肝炎患者中的感染状况及临床意义。方法 采用套式PCR法检测50例非甲-庚型肝炎患者血清标本中TTV DNA,并对其中一株TTV的部分基因序列进行了测定。结果 在50例肝炎患者中,TTV DNA阳性检出率为28.0％(14/50)。TTV序列分析与日本株的同源性为98％。结论TTV感染可能是非甲-庚型肝炎的病因之一。

50 serum samples from patients with nonA - G hepatitis were detected for TTV DNA by nested - PCR method. Partial gene of a TTV isolate was directly sequenced.The results showed that 14 of 50(28% )were positive for TTV DNA.The results suggest that TTV is associated with acute,chronic and fulminant hepatitis,and TTV infection would be the important cause of nonA- C hepatitis.