大鼠视神经损伤后一氧化氮对视网膜功能的影响

Expression of inducible Nitirc Oxide Synthase in retina after the optic nerve injury

目的观察视神经损伤视网膜诱生型一氧化氮合酶 (iNOS)的表达，探讨一氧化氮 (NO)在视神经损伤所致的视网膜节细胞迟发性死亡中的作用 [1]。方法采用大鼠球后视神经横断伤模型，利用免疫组化及计算机图像分析法。结果对照组大鼠视网膜 iNOS表达阴性，视神经横断伤后 1～ 14d视网膜 GCL及 INL出现 iNOS阳性表达，且 iNOS表达在损伤后 7d及 14d明显高于 1d(P< 0.05)。结论视神经损伤诱导视网膜 iNOS的表达，提示视神经损伤早期视网膜 NO的合成增高， NO可能参与介导了视网膜节细胞的迟发性死亡。

Objective To observe the expression of inducible Nitric Synthase(iNOS)in retina after the optic nerve injury.The role NO in delay death of RGCS caused by optic nerve injury.Method Intraorbital optic nerve axotomy model of rat was used to detect the expression of iNOS by immunohistochemistry.The immunlhistochemisttry staining was measured through automatic image analysis technology.Result The expression of iNOS was not edtected in retina of normal and sham－ injury groups,but iNOS positive cells were found in GCL and INL of retina after the optec nerve injury,the espression of iNOS in 7d and 14d were obviosly higher than that in 1d(P< 0.05) .Conclusion Optic nerve injury induces the expression of iNOS in retina,suggesting NO synthesis increase in early stage of the optic nerve injury,it may be one of factors that mediate to the delay death of RGCs after optic nerve injury.