碱性成纤维细胞生长因子对成骨细胞与多孔生物材料黏附特性的影响

The influence of basic fibroblast growth factor on the adhension of osteoblasts to the porous biomaterial

目的 研究碱性成纤维细胞生长因子（ Basic Fibroblast Growth Factor, bFGF）对成骨细胞与多孔生物材料粘附特性的影响。 方法 分别用 0.1、 1、 10、 100、 1 000 ng/ml系列浓度的 bFGF诱导培养基诱导培养成骨细胞 24 h后，接种成骨细胞于多孔生物材料上，测定接种后 1 h的粘附细胞率。结果 bFGF在 0～ 10 ng/ml范围内能促进细胞在材料上的粘附，并在 10 ng/ml浓度下达到最大粘附率（ P< 0.05），当 bFGF浓度进一步增加时，细胞粘附率不再增加，在 1 000 ng/ml时细胞粘附率与对照组无明显差异。结论 10 ng/ml的 bFGF诱导可明显促进成骨细胞与多孔生物材料间的粘附。

Objective To investigat the influence of basic fibroblast growth factor on the adhension characteristics of osteoblasts.Methods 0.1,1,10,100,1 000 ng/ml bFGF were used to induce osteoblasts came from rabbit bone marrow stromal cells for 24 hours. The attached cells was calculated with fluorescence method at 1 h after seeding . Result The number of attached cells had increased after the cells were induced by bFGF when the concentration below 10 ng/ml, and reached the highest number when induced by 10 ng/ml bFGF (P< 0.01). While the number didn't increase with the increase of bFGF concentration. There were no differences when induced by 1 000 ng/ml bFGF compared with the control group. Conclusion The induction of 10 ng/ml bFGF can significantly promote the adhension of osteoblasts to porous biomaterial.