瘢痕形成的分子机制初探:瘢痕组织与成人正常皮肤中EGFR与FGFR2表达差异的对比

A comparison study of EGFR and FGFR2 in normal skin and scar tissue

目的观察瘢痕组织及正常皮肤中的表皮生长因子受体与成纤维细胞生长因子受体在细胞膜上表达的差异。方法标本来自烧伤后需做整形手术的病人，对照选自同一病人的供皮区。选用表皮生长因子受体（EGFR）与成纤维细胞生长因子受体（FGFR）的特异性抗体，对皮肤标本进行免疫组织化学染色，光镜下观察它们的变化。结果正常皮肤与瘢痕组织中都有表皮生长因子受体与成纤维细胞生长因子受体染色阳性的细胞，而瘢痕中的数目明显多于正常皮肤中的表达。结论瘢痕组织和正常皮肤中，表皮生长因子受体染色与成纤维细胞生长因子受体染色存在的差异是造成瘢痕愈合的重要因素之一。

：Objective To observe the expression characteristics of EGFR and FGFR2 between normal skin and scar tissue, in an attempt to probe the molecule mechanism of scar formation. Method The samples of scar tissue and normal skin were taken from incised scar and donor site of patients undergoing plastic operation in our burn unit, respectively. EGFR and FGFR2 were used as the biochemical markers for reparative cells. Immunohistochemistry staining technique was employed to monoclonal antibody to determine the expression of different epithelial cells markers. The positive cells of normal skin and scar tissue were investigated with light microscope. Result There were EGFR－and FGFR2－antibody immunoreactivition positive cells in normal skin and scar tissue, but the protein levels of EGFR－and FGFR2 expression have apparent difference, the number of immunoreactivition positive cells were found in scar tissue were stronger than in normal skin. Conclusion There are the inherent differences of EGFR and FGFR2 antibody immunoreactivition positive staining in cells of scar tissue and normal skin, membrana receptor may be play an essential role of cicatricial healing.