羟基磷灰石涂层高分子人工骨细胞毒性研究

Evaluation on cytotoxicity of HA coated artificial bone

目的研究羟基磷灰石涂层分子人工骨的细胞毒性。方法按照标准方法制备材料浸提液,采用小鼠成纤维细胞(L929)体外细胞培养法,应用形态观察法、MTT法,定性、定量地研究这种人工骨对细胞生长、附着及增殖的影响,并对其细胞毒性作以评价。结果培养细胞形态正常,细胞数量和活性与对照组相比无明显差异,材料对培养细胞附着及增殖无任何不良影响,细胞毒性级为0级。结论人工骨细胞相容性良好,是一种安全的、较有应用前途的骨替代材料。

Objective Cytotoxicity is an important indicator to the biocompatibility of biomaterials.In this study, the cytotoxicity of HA coated artificial bone was evaluated by the method of cell relative growth rate in vitro using L-929 mouse fibroblast.Method The effects of an artificial bone which polymer with HA coating and fiber liners on the proliferation of mouse fibroblast L929 cells were investigated using a tetrazolium based colorimetric assay(MTT assay). 5 different concentration of maceration extract of HA-coated bone were added into culture plate. The samples were tested at 2,4 ,7days respectively after the addition of extract.Result The maceration extract of the artificial bone did not inhibit the cell growth. Scanning electron microscopic observation revealed that L929 cells growing showed normal morphology. The toxicity graduation of the artificial bone was 0-1.Conclusion The results indicate that the artificial bone is compatible with the tested cells. The artificial bone had no cytotoxicity.