宫颈鳞癌患者组织自发性凋亡和辐射诱导凋亡与放疗疗效的关系

Study on the precedence between spontaneous apoptosis and radiation-induced apoptosis for predicting radiotherapeutic effect of cervical carcinoma

目的探讨宫颈鳞癌患者组织细胞自发凋亡和放疗10Gy后辐射诱导的凋亡与放疗疗效的关系,快速确定宫颈鳞癌的辐射敏感性,为肿瘤放射治疗方案的个体化提供理论依据。方法采用核酸电泳和TUNEL方法检测宫颈癌活检组织中细胞凋亡水平。结合病人接受根治性放疗后的疗效,分析细胞凋亡指数与放疗疗效的关系。结果核酸电泳,在放疗后的宫颈癌组织出现典型的DNA梯状带(DNAladder)。TUNEL标记发现,照射前宫颈癌组织存在较低的细胞凋亡指数,但与其放疗的疗效具有相关性;放疗10Gy后凋亡指数(AI10)明显增加(P<0.01),但未观察到与放疗疗效的关系。结论自发性凋亡指数(AI0)可作为宫颈鳞状细胞癌放疗疗效的预测指标。

Objective To investigate the relationship between spontaneous apoptosis,radiation reduced apoptosis and radiotherapeutic effects in squamous cell carcinoma of the uterine cervix,determine the radiosensitivity of cervical carcinoma rapidly and provide the evidence for individual radiotherapy.Method Apoptosis in 35 biopsy samples from patients of cervical squamous cell carcinoma was detected with agarose gelelectrophoresis and TUNEL reaction.All patients were treated with radiotherapy.The radiotherapeutic effects in these patients were observed and the relationship between apoptotic index and clinical prognosis was analyzed.Result A typical DNA ladder was found with DNA electrophoresis for the cervical carcinoma after irradiation,the lower spontaneous apoptosis and ghgher radiation reduced apoptosis were detected by TUNEL reaction in the squamous cell carcinoma of uterine cervix,but only spontaneous apoptotis hsa the obvious correlation with radiotherapeutic effects(P< 0.01).Conclusion The spontaneous apoptosis have the precedence for predicting readiotherapeutic effects,therefore,AI0 may be a useful predictor of response to radiotherapy in carcinoma of the cervix.