飞行员血管紧张素转换酶基因插入/缺失多态性及其血清水平的研究

A study of insertion/delation polymorphism of the angiotensin-converting enzyme gene and serum ACE concentration in pilots

目的 了解飞行员血管紧张素转换酶 (ACE)基因插入 /缺失 (I/ D)多态性及其血清水平 ,探讨 ACE基因多态性与飞行员耐力可能的关系。 方法 飞行员 118例 ,健康对照者 96例 ,用聚合酶链反应 (PCR)扩增技术检测 ACE基因 I/ D多态性 ,用比色法测定血清 ACE水平。 结果 位于 ACE基因第 16内含子的 I/ D多态性经 PCR扩增后呈三种基因型 :纯合子插入型 (II)、纯合子缺失型 (DD)和杂合子插入 /缺失型 (I/ D)。飞行员组 II基因型 (4 4.0 7% )和 等位基因频率 (0 .6 5 )显著高于健康对照组 (分别为 31.2 5 %和 0 .5 2 )。 ACE基因多态性与血清 ACE水平明显相关 (DD>ID,DD>II)。 结论 ACE

Objective To understand insertion/delation (I/D) polymorphism of the angiotensin converting enzyme (ACE) gene and serum ACE concentration in pilots, and to explore the relationship between ACE gene I/D polymorphism and the perfomance of the pilots. Methods The study population consisted of 118 pilots and 96 healthy subjects as controls. The genotypes for an I/D polymorphism in intron 16 of the ACE gene were determined by the polymerase chain reaction (PCR). The serum ACE concentration was measured by high performance liquid chromatography with the use of an artificial substrate. Results The I/D polymorphism in intron 16 of the ACE gene was categorized into three genotypes: two deletion alleles (genotype DD), heterozygous alleles (genotype ID), and two insertion alleles (genotype II). The genotype II and I allele frequency were significantly higher in pilots (44.07% and 0.65) than that in healthy subjects (31.25% and 0.52). The ACE gene I/D polymorphism is strongly associated with serum ACE levels(DD>ID, DD>II). Conclusion It is suggested that I gene of ACE may play a role in perfomance of the pilots.