胰岛细胞瘤特殊类型的电镜观察与免疫组化对照研究

The observation of special types of islet cell tumor and control study of immunohistochemical

目的 通过对胰岛细胞瘤特殊类型的观察 ,研究不同类型的神经内分泌颗粒与临床症状的关系。 方法 采用免疫组化、透射电镜及图象分析的方法。 结果 免疫组化为 Cg A(+)、Syn(+)、胰岛素 (- )、胰高糖素 (- )、胃泌素 (- )、胰多糖 (- )、生长抑素 (- )及胰多肽 (- ) ;电镜下瘤细胞内分泌颗粒主要有两种 ,一种为 30 0 nm以上 ,电子密度低 ;另一种为 15 0～ 170 nm具有致密核心。 结论 神经内分泌颗粒直径的大小与临床症状有关 ,非功能性胰岛细胞瘤多为恶性 ,肿瘤直径>5 cm可以出现功能 ;

Objective The relationship of different types of neuroendocrine granules and clinical symptoms were studied by observed of special types of islet cell tumor. Method Immunohistochemical(IHC),transmission electron microscopy(TEM) and imaging analysis were used. Result IHC: CgA(+), Syn(+), isulin(-),glucagon(-), gastrin(-),pancreatic polyhexose(-),somatostatin (-),and pancreatic polypeptide (-);Two granules were observed by TEM,one kind of granule was more than 300 nm and low dansity, another kind was 150～170 nm and had compact cores. Conclusion The diameters of neuroendocrin granules are related with clinical symptoms,unfunctional islet cell tumor is most malignant, the functions may be appeared when the diameter of tumor >5 cm ;Imaging analysis system has the significance in differentiating between shapes and functions.