利用重型肝炎患者的置出液进行生物人工肝的体外测试

Research of bioartificial liver with exchanged plasma from patient with severe hepatitis in vitro

目的 探讨重型肝炎患者的置出液在生物人工肝研究中的应用价值。方法 以血浆置换手术中无菌收集的重型肝炎患者的置出液作为重型肝炎模型,在体外研究了猪肝细胞型生物人工肝对量出液生化指标及药物转化代谢的影响。结果 重型肝炎患者的置出液经过猪肝细胞型生物人工肝的处理后,置出液中的总胆红素、谷氨酰转肽酶、胆碱酯酶、血糖、胆固醇及糖化血红蛋白的水平出现下降,总蛋白、白蛋白、甘油三脂水平增加,加入置出液中的氨茶碱浓度迅速下降。结论 重型肝炎患者的置出液能反映出生物人工肝对糖、蛋白质、脂肪等物质代谢产生的影响及对药物的转化代谢活性。重型肝炎患者的置出液可作为重型肝炎模型,在研究生物人工肝的疗效研究中具有重要的应用价值。

Objective To study the effect of bioartificial liver on severe hepatitis with the plasma exchanged from the patient with severe hepatitis and the role of exchanged plasma in the research of bioartificial liver as a model of servers hepatitis.Methods The plasma was aseptically collected from the patient with severe hepatitis during the treatment of plasma exchange operation.The effect of bioartificial liver with porcine hepatocytes on the biochemical changes and biotransforma-tion of aminophylline was investigated by the circulating of the plasma in vitro.Results The levels of Mai serum bilirubin,r- glutamyl transpeptidase,cholinesterase,glucose,cholesterol,serum glycoprotein in the plasma from the patient with severe hepatitis were lowed and the levels of total protein,albumin,triglycerides were elevated after the treatment of bioartificial liver by circulating in vitro.The content of aminophylline was decreased rapidly after the treatment.Canduskm The bioartificial liver with porcine hepatocytes could cause the metabolic changes of sugar,lipids and protein and drug biotrans-formation in the exchanged plasma.And the exchanged plasma from the patient with severe hepatitis is sensitive to the treatment of bioartificial liver as a model of severe hepatitis in vitro.It is valuable to the study of the bioartificial liver on severe hepatitis.