病历中检验单的HBsAg和HBV DNA检测

Detection of HBsAg and HBV-DNA on the laboratory test report in medical records

目的 调查保存在传染病医院信息科内的病历中检验申请单被HBV污染情况及其是否具有传染性。方法 随机抽取我院信息科内近 30年的病历 15 0份 ,按每间隔期 10年分为 3组 ,每组 5 0份 ,取每份病历中的检验申请单 ,用标准固体表面HBV采样法取样 ,ELISA法检测HBsAg ,斑点杂交法检测HBVDNA。结果 近 30年的病历中的检验申请单有 16 %～ 5 2 %被HBsAg污染、2 %～ 2 4 %被HBVDNA污染 ;病历保存的时间越长 ,其中的检验申请单HBsAg、HBVDNA检出率越低 (P <0 .0 5 )。结论 传染病医院病历中有相当数量的检验申请单被HBV污染 。

Objective To study if laboratory test report (LTR) had been polluted by hepatitis B virus (HBV) and had infectiousness in the medical records saved by the department of information of an infectious disease hospital. Methods 150 medical records saved by the department of information in the recent 30 years were collected with the random sampling methods. They were divided into three groups by every 10 years, each group had 50 cases. The samples for detection of HBV were collected with HBV standard collection methods from the LTR surface in each medical records. HBsAg and HBV DNA were detected with the ELISA and dot hybridization. Results The rate of the LTR polluted by HBsAg and HBV DNA in the medical records in the recent 30 years was 16%～52% and 2%～24% respectively. For the positive rate of HBsAg?HBVDNA on the LTR, the longer time they saved, the lower positive rate they had;it was statistically significant (P< 0.05 ). Conclusion The LTR had been polluted more badly by HBsAg?HBV DNA in the medical records of the infection hospital. They had definite infectiouness.