前体、诱导子及抑制剂对细胞培养生产紫杉醇的调节作用

REGULATION OF TAXOL PRODUCTION IN CELL SUSPENSION CULTURES OF TAXUS CHINENSIS BY ELICITORS,PRECURSORS AND INHIBITORS

研究了前体、诱导子和抑制剂对中国红豆杉细胞培养生产紫杉醇的影响。结果表明 ,它们之间的协同作用对提高紫杉醇的产量有显著影响。其中向培养基中加入 30mg/L 3 甲基 2 丁烯 1 醇 ,2mmol/L苯甲酸钠 ,10mg/L氯化氯胆碱 (CCC) ,10 0 μmol/L茉莉酸甲酯 (MJ) ,0 1mmol/L丝氨酸 (Ser)可以使紫杉醇含量增加 1141 1%。

The effects of some elicitors,precursors and inhibitors on production of taxol in cell suspensions of T.chinensis were studied.The results showed that their interactions dramatically promoted taxol biosynthesis,and the addition of 30 mg/L 3-methyl-2-buten-1-ol,2 mmol/L sodium benzoate,10 mg/L CCC,100 μmol/L MJ,0 1 mmol/L Ser into the cultures dramatically increased taxol yield by 1141 1%.