摘要
白皮秋花生1012种子萌发3d,获得花生无菌苗,切取子叶、胚轴、子叶柄作为外植体。用农杆菌介导法将外植体侵染20-30分钟转入培养基MS+5mg/L BA+0.5mg/L NAA+100μmol/L AS暗光共培养3d,转至加有280mg/L Cef的培养基P2或P2上诱导芽伸长,长至1-2cm时转至促根培养基MS+0.2mg/L BA+75mg/L Kan+280mg/L Cef上促根,经27天观察有5株再生苗长根。结果表明培养基P2上芽诱导率较高,长势也较好,同时乙酰丁香酮对芽诱导率起促进作用。
Leaflet explants from 3—day—old seeding of white peanut cultivar, we gained unbacterium seeds, cutting down cotyledon, embrgo axis、cotyledon stem、embrgo root via arganagenesis. By agrobacter ium—mediated method. Organagnesis were incubated with LBA4404/ PBI1812 for 20—30 min. Transplanting in MS+5mg/L BA+0.5mg/L NAA+100μmol/L AS for 3 days under dark condition. Then putting organagenesis into two P1 or P2 medium to supplement. The plants were regenerrated, After 27—day, until plants grew 1—2 cm and then. Transplanted in MS+0. 2mg/LBA+75mg/Lkan+280mg/Lcef. The result discovered that five of them formed roots; these plants were more efficient supplement and grew better on the medium of P2 than P1; Acetosyringone stimulate supplement.
出处
《武夷科学》
2003年第1期5-9,共5页
Wuyi Science Journal
基金
国家自然科学基金(398705045)
国家教委留学人员基金
福建省科委(K99007)
关键词
农杆菌
抗线虫基因
花生
遗传转化
agrobacterium tumefaciens
nematode resistance gene
peanut
transformation
