汉族女性峰值骨密度和维生素D受体基因多态性及环境因素的相关性研究

The study of relationships between genetic or environmental factors and the peak bone mineral density in Chinese Han women

目的 研究汉族女性峰值骨密度 (peakbonemineraldensity ,PBMD)与某些环境及遗传因素的关系。方法 选择健康的志愿者 1 5 9人 ,①通过饮食回顾法计算每日钙及蛋白入量 ;②通过 7d运动评估法计算运动强度 ;③使用DEXA骨密度仪 (HologicQDR 4 5 0 0 )测量志愿者腰椎和髋部骨密度 (BMD) ;④应用PCR -限制性片段长度多态性对维生素D受体 (VDR)基因进行多态性分析。结果 ①腰椎BMD结果参照日本人标准 ,低骨量或骨质疏松共有 35人 (2 2 % ) ,正常骨量者为 1 2 4人 (78% )。两组间体重及体重指数 (BMI)间有显著差异 ;②腰椎及髋部股骨颈BMD与体重呈正相关 ,髋部其余位点BMD值和BMI呈正相关 ;③VDR基因BsmI酶切多态性组间BMD无显著性差异。结论 本研究显示 :体重及或BMI是汉族女性PBMD重要保护因素之一。汉族女性PBMD和当前营养状态、运动强度。

Objective To investigate the relationship between the peak bone mineral density (PBMD) and nutrition,physical activity or vitamin D allelic variants in Chinese Han young women Methods One hundred and fifty-nine young healthy women were voluntarily enrolled in the study ①The questionnaire about dietary calcium and protein intake were completed for each subject ②Physical activity levels were estimated with hours per week at various activity levels ③Body mineral density (BMD) was measured using DXEA (Hologic QDR-4500) at lumbar and hip ④The polymorphrisms of VDR gene were detected by PCR-restriction fragment length polymorphrisms assay Results ①The mean PBMD of lumber was 1 025±0 099g/cm 2 Thirty-five (22%) subjects were osteopenia or osteoporosis (group I),and 124 (78%) (group Ⅱ) were within normal BMD range Group Ⅱ presented significantly higher body weight and body mass index (BMI) than that in group I ②The lumbar and the femoral neck BMD were directly correlated to the weight,whereas the other sites of hip BMD to BMI ③There was no significant difference between BMD and VDR genotypes Conclusions Body weight and BMI play important roles to PBMD of Chinese Han women The PBMD at lumber and hip do not related to nutrition and physical activity levels There is no significant difference of BMD levels among VDR genotypes at BsmI cutting sites