前列腺素受体EP_2在子宫内膜癌中的表达

Expression of prostaglandin E2 receptor subtype in endometrial carcinoma

目的 研究前列腺素 (PGE2 )受体EP2 在子宫内膜癌中的表达 ,探讨EP2 在子宫内膜癌中的表达变化与雌激素受体依赖的关系 ,以及在子宫内膜癌的发生、发展过程是否存在非雌激素受体的作用。方法5 0例子宫内膜癌组织 (Ⅰ期 2 9例、Ⅱ期 7例、Ⅲ期 1 2例、Ⅳ期 2例 ,G1级 2 6例、G2级 1 5例、G3级 9例 ) ,正常子宫内膜 1 0例 ,术前未经放化疗及激素等内分泌治疗。应用逆转录 -聚合酶链反应 (RT -PCR)技术 ,半定量研究各组中EP2 的mRNA表达。结果 子宫内膜癌G1组、G2组、G3组均高于正常组 ,差异有统计学意义 (P<0 0 5 ) ,而且随细胞分化越差 ,EP2 受体表达水平呈上升趋势 ,差异有统计学意义 (P <0 0 0 1 ) ;子宫内膜癌Ⅰ期组、Ⅱ～Ⅳ期组均高于正常组 ,差异有显著统计学意义 (P =0 0 0 1 ) ,并随分期变化 ,分期越晚 ,表达值升高 ,期别之间相比差异无统计学意义 (P =0 1 7) ;在子宫内膜癌中 ,雌激素受体表达阴性组比阳性组的EP2表达水平显著升高 ,差异有统计学意义 (P <0 0 0 1 ) ;而孕激素受体表达阴性组与阳性组的EP2 表达相比较差异无统计学意义 (P >0 0 5 ) ;绝经前 (2 0例 )与绝经后 (30例 )患者的子宫内膜癌中EP2 的表达分别为 0 90 5±0 2 5 1和 0 94 1± 0 336 ,二者差异无统计学意义 (P

Objective To explore the relationship between the expression of prostaglandin E2 receptor subtype (EP 2) and etiology of endometrial carcinoma,as well as the possible role of EP 2 in estrogen-independent endometrial carcinoma Methods Expression of mRNA of EP 2 in 50 cases of endometrial carcinoma and 10 normal endometrum was analyzed by reverse transcription-polymerase chain reaction (RT-PCR) Results The mRNA level of EP 2 was significantly higher in endometrial carcinoma than that in normal endometrum ( P <0 05) The higher histological grade of the disease,the more mRNA expression of EP 2 ( P <0 001) The mRNA expression of EP 2 was higher in estrogen receptor(ER) negative specimen than in ER positive ones( P <0 001) Conclusions Increased expression of EP 2 receptor in endometrum may lead to endometrial carcinoma,especially in those cases with negative ER