铽-培氟沙星的荧光特性研究及培氟沙星的测定

Fluorescence Characteristics of Tb^(3+)-Pefeoxacin and Determination of Pefeoxacin

适宜条件下 ,铽 (Ⅲ )与培氟沙星反应极易形成络合比为 1∶2络合物。据此建立了简单、快速、灵敏测定培氟沙星的荧光方法 ,并优选了反应的最佳条件 ,探讨了络合物分子内部能量传递的机理及影响因素 ,结果表明 ,在 0 0 1～ 1 6 7μg·mL- 1 浓度范围内培氟沙星与其荧光强度呈良好的线性关系 ,其线性方程为F=12 0 0 1C +2 6 0 8,检出限为 0 0 1μg·mL- 1 。经样品测定 ,其回收率为 95 2 %～ 10 1 7% ,RSD为 2 9%～3 0 %。该方法可成功地用于不经任何处理的血清及胶囊中培氟沙星含量的测定。

Terbium(III) can react with Pefeoxacin (PEFX) to form 1:2 coordination compound in a near neutral medium. Based on this reaction, we proposed a simple, rapid, and sensitive new method for the fluorimetric determination of Pefeoxacin and investigated the reaction conditions in detail; meanwhile we discussed the efficiency and mechanism of the intramolecular energy transfer. The results show that in the range of 0.01-1.67 mug(.)mL(-1), there is a linear relationship between the concentration of PEFX and its fluorescence intensity, and the regression equation is F = 120.01 C + 2.608, with the detection limit of 0.01 mug(.)mL(-1), the recovery within the range of 95.2%-101.7%, and the RSD of 2.9%-3.0%. This method has been successfully applied to the determination of Pefeoxacin in untreated human serum and gelatine capsule.