丝裂霉素C的给药间隔与膀胱癌细胞耐药性的研究

Study of intervals of mitomycin C treatment and the mutildrug resistance of bladder cancer

目的研究丝裂霉素C(MMC)的给药间隔与膀胱移行细胞癌多药耐药之间的关系及机制。方法分别间隔24、48、72和96小时,用MMC处理膀胱移行细胞癌BIU87细胞株2个小时,共5次,用MTT法检测细胞毒作用,Westernblot分析p170和p53的表达。结果①MMC首次处理BIU87、间隔24、48、72和96小时给药的IC50分别为4.41、0.71、2.83、4.51及6.16μg/ml;②间隔24小时给药,p53显著下调,未检测到p170表达,间隔48、72、96小时给药,p53持续表达,p170的表达逐渐增强。结论膀胱癌细胞的耐药性与丝裂霉素C的给药间隔密切相关,其机制可能与p53表达的变化有关。

Objective To study the relationship between the intervals of mitomycin C treatment and the mutildrug resistance of bladder transitional cell cancer line and its mechanism.Methods The bladder transitional cell cancer line BIU-87 was treated with mitomycin C two hours every time for five times with intervals of 24,48,72 and 96 hours respectively.Cystotoxicity was measured by MTT and p53 and p170 expression were analyzed by Western blot.Results ①The IC_50(μg/ml)were 4.41,0.71,2.83,4.51and 6.16 respectively of the first time treatment,treatment after 24,48,72 and 96 hours.②p53 was significantly down-regulated while p170 was not detected at 24 hours interval treatment.p53 continuously expressed and p170 increasingly expressed at 24,48,72 and 96 hours intervals treatment.Conclusion The mutildrug resistance of bladder cancer cell was closely related with the treatment intervals of mitomycin C,which maybe correlated with p53 expression.