消炎痛对兔脑外伤后脑组织氧自由基反应的影响

Influence of indomethacin on free radical reactions in brain tissues of rabbits with brain injury

目的 :探讨消炎痛对急性实验性脑外伤后兔脑组织氧自由基反应的影响。方法 :30只家兔随机分为A组 (正常对照组 )、B组 (生理盐水治疗组 )及C组 (消炎痛治疗组 ) ,每组 10只动物。其中A组不致脑外伤 ,作为正常对照。B、C 2组采用自由落体打击法建立兔闭合性脑外伤模型。C组于外伤后 10min开始 ,经耳缘静脉缓慢注入消炎痛针剂。B组于外伤后相同时间同样方法注入等量的生理盐水 ,作为生理盐水治疗对照。B、C 2组于外伤后 4h将动物处死 ,完整取出大脑半球以干湿重比较法测定大脑含水量 ;取挫伤灶周边脑组织制成匀浆 ,生化测定脑组织中过氧化物歧化酶 (SOD)活性和脂质过氧化物 (LPO)含量。结果 :B组动物致脑外伤后 4h ,脑组织含水量及LPO含量分别为 (79 918± 1 44 9) %和 (143 5± 2 4 9)nmol g ,较对照组显著为高 (P <0 0 1及P <0 0 5 ) ,SOD活性为(1 97± 0 16 )Nu mg,显著低于对照组 (P <0 0 1)。C组应用消炎痛治疗后 ,脑组织含水量及LPO含量分别为 (78 2 14± 1 315 ) %和 (118 1± 13 7)nmol g ,与对照组比较 ,差异无显著性 (P >0 0 5 ) ;SOD活性为 (2 2 5± 0 2 1)Nu mg ,较B组明显升高 (P <0 0 5 )。结论 :脑外伤早期应用消炎痛治疗 ,可减轻外伤脑组织中的氧自由基反应 ,保护血脑屏障 ,延缓?

Aim: To investigate the effects of indomethacin(IM) on free radical reactions in brain tissues of rabbits with acute experimental brain injury.Methods: Thirty rabbits were divided into three groups randomly(ten animals in each group).Group A:the normal control group.Group B:the control group treated with saline.Group C:the group treated with IM.The animals in group A were not hit.To establish the acute closed brain injury model,the animals of B and C groups were hit by a free falling object.At the loth minute after brain injury,the animals in group B and C were injected with normal saline and IM 4 mg/kg respectively at the first time through the ear edging vein. To evaluate SOD activity, LPO and water content in brain tissues,the animals were killed and their brains were fetched out intactly at four hours after brain injury.Results: The water content and content of LPO in brain tissues of group B animals were (79.918±1.449)% and (143.5±24.9)nmol/g respectively,which were significantly higher than those of the normal control group ( P<0.01,P<0.05),and the activity of SOD was (1.97±0.16)Nu/mg,significantly lower than that in the control group C (P<0.01).After treatment with IM,the values of water and LPO content in group animals were (78.214±1.315)% and (118.1±13.7)nmol/g respectively,no significance was found between group C and group A( P>0.05) .The mean value of SOD activity was (2.25±0.21)Nu/mg,higher than that in group B (P<0.05) . Conclusion: IM treatment at the early stage of brain injury could attenuate oxygen free radical reaction in injured brain tissues,protect blood brain barrier,prevent traumatic cerebral edema being formed and developing further.