肺炎链球菌大环内酯类抗生素的耐药性分析

Characterization of macrolide resistance of Streptococcus pneumoniae from clinical isolates

目的本研究通过对肺炎链球菌耐药表型检测分析,掌握本地区肺炎链球菌耐药的现状和趋势。方法本文用E试验和K-B纸片扩散法检测84株肺炎链球菌临床分离株对9种抗生素的敏感性;用双纸片法确定大环内酯类耐药表型。结果84株肺炎链球菌红霉素耐药占85.7%(72/84),对青霉素不敏感率达57.1%(48/84)。左氧氟沙星、阿莫西林/克拉维酸对该组细菌有较好的体外活性,敏感率分别为83.3%(70/84)和88.1%(74/84)。结论肺炎链球菌对大环内酯耐药严重,且表现对四环素、复方磺胺甲噁唑、青霉素等多重耐药;南昌地区大环内酯类耐药表型主要以cMLSB为主。

Objective To understand the mechanisms of macrolide resistance and resistant phenotypes of Streptococcus pneumoniae in Nanchang . Methods Antibiotic susceptibilities were determined by the E-test method or K-B disk diffusion test according to Clinical Laboratory Standards Institute (CLSI). Macrolide resistant phenotypes were classified using a double disc test with erythromycin and clindamycin discs (Dtest). Results Of 84 isolates, 85.7% (72/84) was resistant to erythromycin, with 57.1% (48/84) of PNSSP. Amoxicillin/clavacin and levofloxacin retained activities against the most of the isolates. Conclusions The most strains in our data are highly resistant to erythromycin, and the most common macrolide resistance phenotype is the cMLSB phenotype in Nanchang.