川硬皮肿腿蜂染色体制备方法探讨

PREPARING METHOD OF CHROMOSOME OF SCLERODERMA SICHUANENSIS XIAO (HYMENOPTERA: BETHYLIDAE)

对川硬皮肿腿蜂Scleroderma sichuanensis Xiao(Hymenoptera:bethylidae Sclerderma)染色体制备方法进行了探讨,结果表明;以川硬皮肿腿蜂雌成虫卵巢为材料,经0．01％秋水仙素水溶液处理10min,蒸馏水低渗5min,用甲醇、冰醋酸(3:1)固定液固定15min, Giemsa染液染色30min,可以获得形态良好、分散适中、着色清晰的染色体。

It discussed the preparing method of chromosome of Scleroderma sichuanensis ( Hymenoptera: Bethylidae Sclerderma ). The result showed that making use of female insect ovaries as material, which were disposed about 10min in 0.01 %colchicine liquor, and were infiltrated 5min in distilled water, then were solidifided about 15min in methanol、glacial acetic acid(3:1),finally, were dyed 30min in giemsa dye, the experiment might attain very clear chromosome in form and colour.