内蒙古地区天然臭柏种群遗传多样性的RAPD分析

RAPD Analysis on Genetic Differentiation of Sabina vulgaris Populations in Inner Mongolia Area

应用随机扩增多态性DNA(RAPD)标记法研究了内蒙古地区臭柏种群的遗传分化,所用的18条引物对4个臭柏种群的55个样本扩增出124个位点,其中多态位点100个,多态率达80.7%。种内的平均Nei's多样性指数和Shannon's多样性指数分别为0.286和0.428,种群内分别为0.232和0.347,基因多样性变化趋势为毛乌素沙地(0.258,0.386)>阴山山脉西部(0.258,0.376)>浑善达克沙地(0.231,0.345)>内蒙古贺兰山(0.184,0.281)。臭柏种群总的基因多样性(Ht=0.288),大于种群间的基因多样性(Hs=0.233)。种群间的遗传分化系数(Gst)为0.183,种群内的遗传变异占总遗传变异的81.7%。臭柏种群间的平均遗传距离为0.086,平均相似性系数为0.918,4个种群之间有比较相似的遗传多样性,由于地理隔离,种群间也存在分化。

Genetic Differentiation of Sabina vulgarisPopulation in Inner Mongolia area was studied with Random Amplified Polymorphic DNA(RAPD) markers.After analyzed by Popgen32 Version1.31,the results were as follows.Through the amplification with 18 random primers,124 repeatable loci in which 100 were poly-morphic were detected.Percentage of polymorphic loci was 80.7%.Nei'sindex and Shannon'sindex of the gene diversitywere 0.286 and 0.428 in special,and were 0.232 and 0.347 within populations respectively.The genetic diversities of each population were Mu Us sandland(0.258,0.386)>Yinshan mountain(0.258,0.376)>Hunshandake sandland(0.231,0.345)>Helan mountain(0.184,0.281).The genetic differentiation was Gst=0.183.The genetic variation among populations was 18.3%,and within population was 77.63%.The average genetic distance among populations was 0.086 and Nei's genetic identity was 0.918.The genetic diversities in 4 populations were similar,but the genetic differentiation existed because of geographic isolation.