ddy小鼠替代大鼠的经济简便的抗焦虑药筛选改进模型——间氯苯哌嗪诱导的小鼠明暗箱焦虑模型

mCPP-induced Anxiety Model in Light-dark Box in ddy Mice ——A New Economic and Simple Method for Screening Anxiolytic Activity

目的 建立一个部分模拟临床病理的简便易得、经济有效的用于筛选和研制抗焦虑药的模型。方法 用间氯苯哌嗪 (mCPP)诱导产生焦虑 ,观察ddy小鼠和ICR小鼠在明暗箱的行为表现。结果 mCPP在SC 1- 4mg kg的剂量下即可显著降低ddy小鼠在明箱的活动次数 ,而对暗箱的活动次数影响不显著 ;1mg kg与 4mg kg组比较 ,在明箱的活动次数无显著差异 ;mCPP在SC 2 - 10mg kg的剂量下对ICR小鼠在明箱的的活动次数影响不太显著 ,只在10mg kg剂量下才显著 ;而对暗箱的活动次数却影响较大 ,在 2mg kg的剂量下即可显著降低活动次数。用安定对该模型进行验证 ,发现只用较小的样本量即可得出显著结果。结论 用ddy小鼠可以取代Wistar大鼠建立mCPP诱导焦虑的明暗箱模型 ,在样本量较小的情况下即可得到有效结果。所以是一个能够克服大鼠实验缺点的、简便易得且经济有效的用于筛选和研制抗焦虑药的良好模型。但用mCPP诱导的焦虑模型对小鼠的种属有选择性 ,ICR小鼠不适于上述模型。

Objective To develop a new partially clinical mimic, economic and simple anxiety model for screening and studying anxiolytic model. Method observing the behavior activity of DDY and ICR mice in Light_Dark box after SC mCPP to induce anxiety. Result It can be significantly decrease the activity of ddy mice in light box after sc 1-4mg/kg, but the activity in dark box is not significantly changed. There is no significant difference in the light box between the 1mg/kg and the 4mg/kg group. mCPP has little effect on the activity of ICR mice in the light box, but it can significantly decrease the activity in the dark box between 2-10mg/kg. It is easy to prove the anxiolytic effect of Diazepam only in a small sample by using this model. Conclusion It is easy to get the effective result by using only small sample in the Light_Dark box after mCPP_induced anxiety in ddy mice, so we suppose it is partially clinical mimic, economic and simple anxiety model for screening and studying anxiolytic model.