实验昆虫实验动物化的初步研究

Preliminary Research on Experimental Animalization of Experiment Insect

With the development of science and technology, life science is involving in many fields. The requirements for the methods of experiment and experiment object are more abroad, more strict and more diversified. As laboratory animals of experimental material in the life science, great changes have taken place since then. As for the exploitation of new kind of laboratory animals, it is not only limited in experimental Animalization of ordinary mammals but also expands steadily from Experimental Animalization of birds, reptiles, fish and invertebrates. Now insects of invertebrate have been used in life science research and are enriched gradually in application fields. China is one of the earliest countries that exploits and makes use of insect resources in the world and is also the country with the largest insect resources on the earth. At the present, the insect resources are studied, developed and utilized in many fields and on all levels regarding the traditional raising as its foundation and the biological technology as the guide. The scope of insect research expands from insect body to the function, microorganism, and the use of insect gene, which is developed widely in the fields of agriculture, medicine and industry. In agriculture, the biologic pesticides have been researched, developed and utilized since 1960’s in our country. The biological prevention of our country has ranked among the advanced countries. With the further development of research of biological prevention and cure, the experiment insects, acting as experiment material used in biologic pesticide, are playing important roles in pathogenic filtration virus’s multiplication, measurement of toxicity and mould of insects etc. Therefore, the experiment insects’ house raising, standardization of condition control, legalization and standardization of management, and socialization of production and supply are the developing trends of biologic prevention and cure and development of other fields of life sciences. According to the raising experience, existing conditions, human resources and some other groundwork of Wuhan la boratory animal center, we have made progress in the preliminary study of experiment insect’s animalization which mainly involving in lepidopteron such as ,bollworm, beet noted, fleck noctuid . According to the requirement of standards of quality and environment of experiment insects of our country and combining the design and present conditions of our labortory animal building , we have improved the environment and facilities of the experimental insects room and have controlled reasonably the sunshine, temperature, humidity, ventilation , fitful feeding (cage) shelf and microbes surroundings in and out door, which are different for insects in their different phases of growth so as to make indoor environment to meet the breeding demands of experimental insects. On the basis of meeting the lab’s needs of ordinary scientific research and development, we have mapped out stable condition monitoring standards for experiment insects. We have made efforts to map out other standards with regards to the research of artificial feeding stuff for lepidopteron; counting and measurement of nutrition percentage; improvement of technology relating to processing and storing, etc. so that these measures will ensure the experiment insect’s high quality and normal reproduction. Presently, bollworm’s continuous in door block out reproduction have reached thirty generations, while beet noctuid reached twenty five generations with stable capability of production and supply scale. We can supply ten thousand new bollworms for the experiment purpose per day. On the basis of standardizing the experimental condition and house raising, we will develop further the inspection experiment of physiological, biologic and chemical function index and hereditary feature to microbes and vermin of the experimental insects so as to map out a relevant monitoring quality standard for providing life sciences with sufficient theoretical basis. With the further

With the development of science and technology, life science is involving in many fields. The requirements for the methods of experiment and experiment object are more abroad, more strict and more diversified. As laboratory animals of experimental material in the life science, great changes have taken place since then. As for the exploitation of new kind of laboratory animals, it is not only limited in experimental Animalization of ordinary mammals but also expands steadily from Experimental Animalization of birds, reptiles, fish and invertebrates. Now insects of invertebrate have been used in life science research and are enriched gradually in application fields. China is one of the earliest countries that exploits and makes use of insect resources in the world and is also the country with the largest insect resources on the earth. At the present, the insect resources are studied, developed and utilized in many fields and on all levels regarding the traditional raising as its foundation and the biological technology as the guide. The scope of insect research expands from insect body to the function, microorganism, and the use of insect gene, which is developed widely in the fields of agriculture, medicine and industry. In agriculture, the biologic pesticides have been researched, developed and utilized since 1960's in our country. The biological prevention of our country has ranked among the advanced countries. With the further development of research of biological prevention and cure, the experiment insects, acting as experiment material used in biologic pesticide, are playing important roles in pathogenic filtration virus's multiplication, measurement of toxicity and mould of insects etc. Therefore, the experiment insects' house raising, standardization of condition control, legalization and standardization of management, and socialization of production and supply are the developing trends of biologic prevention and cure and development of other fields of life sciences. According to the raising experience, existing conditions, human resources and some other groundwork of Wuhan la boratory animal center, we have made progress in the preliminary study of experiment insect's animalization which mainly involving in lepidopteron such as ,bollworm, beet noted, fleck noctuid . According to the requirement of standards of quality and environment of experiment insects of our country and combining the design and present conditions of our labortory animal building , we have improved the environment and facilities of the experimental insects room and have controlled reasonably the sunshine, temperature, humidity, ventilation , fitful feeding (cage) shelf and microbes surroundings in and out door, which are different for insects in their different phases of growth so as to make indoor environment to meet the breeding demands of experimental insects. On the basis of meeting the lab's needs of ordinary scientific research and development, we have mapped out stable condition monitoring standards for experiment insects. We have made efforts to map out other standards with regards to the research of artificial feeding stuff for lepidopteron; counting and measurement of nutrition percentage; improvement of technology relating to processing and storing, etc. so that these measures will ensure the experiment insect's high quality and normal reproduction. Presently, bollworm's continuous in door block out reproduction have reached thirty generations, while beet noctuid reached twenty five generations with stable capability of production and supply scale. We can supply ten thousand new bollworms for the experiment purpose per day. On the basis of standardizing the experimental condition and house raising, we will develop further the inspection experiment of physiological, biologic and chemical function index and hereditary feature to microbes and vermin of the experimental insects so as to map out a relevant monitoring quality standard for providing life sciences with sufficient theoretical basis. With the further development