甲基叔丁基醚对大鼠血清生化指标及肾超微结构的影响

The Effect of Methyl Tertiary Butyl Ether on Rat Serum Biochemical Index and Ultra Structure of Nephric Cortex Cells

[目的 ]甲基叔丁基醚 (MTBE)是一种新型的汽油添加剂 ,常用来提高汽油辛烷值和减少汽车尾气中有害物质的排放。它的毒性研究国内外学者的结论尚不一致。随着国内MTBE的使用推广 ,有必要了解国产MTBE的毒性。[方法 ]SD大鼠经灌胃给MTBE 10 0 0、6 0 0、2 0 0mg kg ,每天一次 ,每周 5d ,共 13周亚慢性染毒。采用生化分析仪分析了大鼠相关血清生化指标的改变。并用电镜观察了大鼠肾皮质区超微结构的变化。 [结果 ]血清生化分析结果表明各剂量组动物乳酸脱氢酶 (LDH)和谷草转氨酶 (AST)有明显下降 ,但仍在正常范围内。其他指标如谷丙转氨酶 (ALT)、血清总蛋白 (TP)、白球蛋白比值 (A G)、尿素氮 (BUN)、肌酐 (Cr)未见明显改变。电镜观察到肾小管细胞胞浆内有初级溶酶体、线粒体异常增殖 ,10 0 0mg kg高剂量组中肾小球系膜基质与足细胞足突肿胀现象。 [结论 ]肾脏细胞对MTBE的毒作用处于一种代偿状态 ,血清生化指标未见改变 。

Methyl tertiary butyl ether(MTBE) is a new gasoline additive used to increase gasoline octane value and decrease the exhaust of some harmful pollutants,but there is still debate on its toxicology. With the expansive use of MTBE in China,it is imperative to explore homemade MTBE toxicology. In this artcle,subchronic oral MTBE assay was carried out on Sprague Dawley rats,the doses were 1 000 mg/kg,600 mg/kg,200 mg/kg and 0 mg/kg body weight,once daily,five days weekly,for 13 weeks. The serum biochemical indexes were detected,meanwhile the damage of ultra structure of nephric cortex cells was observed with electron microscopy. The biochemical index including LDH,AST,ALT,TP,A/G,BUN and Cr were analyzed,the results showed that there was no significant difference between test and control groups except a distinct decrease of LDH and AST,but they are within the normal level. The ultra structure was characterized by appearance of primary lysosomes,proliferation of mitochondrion as well as swell of podocyte and base membrane. [Conclusion] The damage is still in the degree of compensation,and no obvious difference of serum enzymes and proteins is observed. There was no appearance of damage on the function of kidney.