人大肠癌转移与Ⅳ型胶原酶的关系

The Relationship of Metastasis with Type Ⅳ Collagenases in Human Colorectal Cancer

目的 探讨人大肠癌转移与Ⅳ型胶原酶的关系。方法 应用明胶酶谱分析法 (Zymography)检测了 31例大肠癌组织Ⅳ型胶原酶的活性 ,并用RT PCR方法检测 92kDⅣ型胶原酶RNA的表达情况。结果 在伴有转移的大肠癌标本中 ,Ⅳ型胶原酶的活性形式与非活性形式表达均较高 ,92kDⅣ型胶原酶的mRNA与在转移癌中高表达。结论 Ⅳ型胶原酶与大肠癌的转移密切相关。

Objective To investigate the relationship of metastasis with type Ⅳ collagenases in human colorectal cancer Method Quantitatively detect activity of type Ⅳ collagenases by gelatinembedded SDS-PAGE and study 92 kD type Ⅳ collagenases gene expression by RT-PCR method in 31 cases of colorectal cancer Result The activity of both active and latent forms of type Ⅳ collagenases in metastatic cases is higher than that of of non-metastasis ones, and 92 kD type Ⅳ collagenase gene expression was also high in accordance with its activity Conclusion The metastasis of human colorectal cancer is closely related to type Ⅳ collagenases