中国对虾类淋巴组织培养中细胞异常的病理研究

The Pathological Observation of Shrimp Penaese chinensis Lymphoid Tissues Showing Abnormal in Tissue Culture

在对养殖中国对虾和海捕中国对虾的类淋巴 器官进行组织培养时，发现迁出的细胞空泡化、细胞内颗粒较多。培养一周后由养殖中国对 虾类淋巴组织迁出的细胞开始大量脱落，而由海捕中国对虾类淋巴组织培养出的细胞可生长 30d左右。对未经培养的类淋巴组织及培养的类淋巴组织进行透射电镜观察，发现在养殖中 国对虾类淋巴组织的细胞中除了有球形病毒及球状和多面体状病毒包涵体外，在细胞质中还 含有大量类立克次氏体样原核生物，而且每个细胞中的类立克次氏体均由一单层膜包在一起 ；在海捕中国对虾类淋巴组织的细胞中也发现有球形病毒及球状病毒包涵体，另外还在少量 细胞中发现有类立克次氏体样原核生物存在。感染病毒的组织细胞呈现空泡化、核膜肿胀破 裂、核质固缩、高尔基体肿胀、线粒体嵴消失等病理变化。感染有类立克次氏体样原核生物 的细胞不同程度地呈现出细胞器消失、核染色质固缩等病理结构。

Tissue culture of farmed and wild shrimp (Penaes e chinensis) was studied. The vacuolization of migrated cells from cultured ti ssue and many granules in them were observed. These attached cells of farmed shr imp gradually came off after 7 days culture. The cells of wild shrimp could be a live for 30 days or so. We examined the lymphoid tissues of shrimp and cultured tissues in vitro with transmission electronic microscope (TEM). Besides a ki nd of spherical virus and two kinds of virus inclusion, a large number of Ricket tsia-like organisms were found in the lymphoid tissues of farmed shrimp. While in the lymphoid tissues of wild shrimp, a few spherical viruses, a kind of globa l virus inclusion and Rickettsia-like organisms were also found in a few cells. But some cells of wild shrimp infected by the Rickettsia-like organisms showed pycnosis. Pathological changes, including vacuolization, nuclear membrane hyper trophy, nuclear chromatin pycnosis, Golgi body swelling, cristae of mitochondria disappearing were observed in cells infected by virus. Those cells infected by Rickettsia-like organisms showed organelle disappearing and chromosome pycnosis .