摘要
参照Isabel方法用不同浓度的环丙沙星(Ciprofl oxacin)，处理两株革兰氏阳性菌；北京棒状杆菌(Corynebacterium pakinense);枯草芽 孢杆菌(Bacillus subtilis)和两株革兰氏阴性菌：霍乱弧菌(Vibrio cholerae)和大肠杆 菌 O157：H7(Escherichia coli O157:H7)。结果发现经乳酸环丙沙星处理后的细菌形 态均变长变大，计数结果表明环丙沙星处理的细菌的活菌直接计数与用萘啶酮酸(Nalidixic acid)处理的活菌直接计数法(DVC)结果相似，高于平板菌落计数结果，而且对部分革兰氏 阳性菌也可应用此法进行活菌直接计数。
Four bacteria including two G＋ bacteria-Corynebacteri um pekinense and Bacillus subtilis and two G－ bacteria-Vibrio cholerae and Es cherichia coli O157:H7 were treated with different concentrations of Ciprofloxac in lactate resulted in larger and longer bacteria than normal.The viable bacteri al count recorded by Ciprofloxacin lactate acting as inhibitor of cell divesion was similar to that of Nalidixic acid method.It was also found that viable G＋ bacteria counted in this way showed a better result than the conventional plate couting method.
关键词
环丙沙星
活菌直接计数法
革兰氏阳性菌
革兰氏阴性菌
ciprofloxacin
direct viable count
gram-positive bact eria
gram-negative bacreria姚斐，女，1972年出生，硕士。
