酵母双杂交系统的应用及发展前景

Yeast Two-Hybrid Systems, Their Applications and Beyond

酵母双杂交系统有利于检测蛋白质与蛋白质、蛋白质与DNA 、蛋白质与RNA和蛋白质与小分子之间的相互作用，因而对分子调控体系的研究起巨大的反 作用。文中系叙述了基于Ga14系统的双杂交技术的应用及其局限性，还介绍了单杂交系统、 双杂交系统、三杂交系统以及Sos恢复系统(SRS)。与其它领域一样，酵母双杂交系统将会在 海洋分子生物学研究中取得成功。

Yeast two-hybrid systems have contributed ver y significantly to the unraveling of molecular regulatory networks by facilitati ng the discovery of protein-protein, protein-DNA, protein-RNA and protein-sm all molecule interactions. In this article, we describe the two-hybrid technolo gy, mainly based on the Gal4-based system, and its applications and limitations . We also introduce several variations of the two-hybrid systems, i.e. one-hyb rid, three-hybrid and reverse two-hybrid systems for different applications. F inally, the Sos recruitment system (SRS) is presented. Judging from the robust s uccess in other fields, application of yeast two-hybrid systems and their varia tions to marine molecular biology will promise to have significant discoveries.