黄芩茎段愈伤组织诱导和再生植株的研究

Research on Inducing Callus from Scutellaria Baicalensis Georg Stem and Plantlet Regeneration

本实验以黄芩的茎段为外植体,在附加不同植物激素组合的培养基中对愈伤组织的诱导和植物再生进行研究。结果表明:采用修改的Ms培养基(除去甘氨酸,维生素B_1含量加至0.5mg/L,pH5.7)附加2mg/L2,4-D;可由黄芩的茎段诱导大量胚性愈伤组织;愈伤组织继代选用0.5mg/L2,4-D为宜;不定芽的诱导采用Ms培养基(20g蔗糖,8g琼脂,0.1水解干酪素)+6-BA(0.5mg/L)+IBA(0.2mg/L);幼苗进一步转接至1/2MsIBA(0.5mg/L)生根培养基中,可完成黄芩茎段愈伤组织诱导和再生植株的培养过程。

Ihe callus induction and plantlet regeneration were researched on the different plant hormone com- bination.Media with Scutellaria baicalensis Georgi stem as explants.The results showed as below:Scutellaria ba- icaensis Georg Callus were induced on the modified Ms containing vitamin B10.5mg/Land 2,4-D2mg/Lwithout glycin,pH5.7 The scutllbria baicalensis Georgi callus were Subcuitured on the modified,Ms containing 2,4- D0.5mg/L.The plantlet regeneration were performed on the MS mtolium continuing sucrose 20g,...