血管内皮生长因子在肝细胞癌及慢性肝病检测中的应用

Clinical significance and detection of serum vascular endothelial growth factor in patients with hepatocelluar carcinoma and chronic hepatitic diseases

目的探讨血清中血管内皮生长因子(VEGF)与肝细胞癌及慢性肝病的关系。方法采用双抗体央心ELISA法对43例肝细胞癌、23例乙型肝炎肝硬化、36例慢性乙型肝炎患者血清中VEGF进行了检测,并与30例健康者对照。同时检测了10例转移与33例非转移肝细胞癌患者血清VEGF浓度并对11例肝细胞癌患者化疗前后血清VEGF浓度进行了比较。结果肝细胞癌、肝炎肝硬化和慢性肝炎患者血清VEGF浓度明显高于正常对照组(p值<0.001,P值<0.01、P值<0.01),并且肝细胞癌组明显高于肝炎肝硬化和慢性肝炎组(p值均小于0.01);肝癌转移患者血清VEGF浓度显著高于非转移者(p<0.01),肝细胞癌患者化疗(有效)后血清VEGF浓度较化疗前明显降低(p<0.05)。结论检测肝细胞癌及慢性肝病患者血清VEGF含量在一定程度上反映了血液VEGF在肝细胞癌新生血管形成中的意义和在慢性肝病发病中的作用,并可作为观察肝细胞痛有无发生转移的参考指标。另外,检测血液VEGF水平对判断肝癌化疗效果有一定参考价值。

Objective To investigate the relation between the vascular endothelial growth factor(VEGF) and the hepatocelluar carcinoma(HCC) and chronic hepatitic diseases.Methods Serum VEGF were detected in 43 patients with hepatocelluar carcinoma,23 liver cirrhosis.36 chronic hepatitis B and 30 normal controls by ELISA,10 metastased HCC and 33 non-metastased HCC were also detected.Serum VEGF levels before and after chemotherapy in 11 patients with hepatocelluar carcinoma were compared.Resuits The patients with hepatocelluar carcinoma(p<0.001),liver cirrhosis(p<0.0 1) and chronic hepatitis B(p<0.0 1) showed higher levels of serum VEGF than normal controls;hepatocelluar carcinoma showed higher levels of serum VEGF than liver cirrhosis(p<0.01)and chronic hepatitis B(p<0.01);metastased HCC showed significantly higher levels of serum VEGF than nod-metastased HCC(p<0.0 1);serum VEGF levels in patients with HCC after chemotherapy were lower than that before chemotherapy(p<0.05).Conclusion VEGF might play an important role in the growth of new blood vessels in patients with hepatocelluar carcinoma and the pathogenesis of chronic hepatitic disease.Serum VEGF levels might serve as an diagnostic index for hepatocelluar carcinoma and an reference index for estimating if hepatocelluar carcinoma metastased.