日本血吸虫成虫脲溶42kD蛋白的分离及其初步诊断应用

Fractionation of 42 kD Protein from Urea-soluble Schistosoma Japonicum Adult Worm Antigens and Its Primary Use for Schistosomiasis japonica Diagnosis

目的 :分离日本血吸虫成虫脲溶抗原中有免疫学活性的抗原分子 ,探讨其诊断血吸虫病的效果。方法 :利用SDS PAGE及免疫印迹法分析日本血吸虫成虫脲溶抗原 ,采用电泳层析法分离有免疫活性的 4 2kD蛋白 ,ELISA分析 4 2kD蛋白诊断血吸虫病的灵敏度和特异性。结果 :电泳层析获得了具有免疫学活性的 4 2kD蛋白 ;分离的 4 2kD蛋白诊断血吸虫病的灵敏度和特异性分别为 88.0 9%和 97.0 6 %。结论 :日本血吸虫成虫脲溶 4 2kD蛋白是一种日本血吸虫的血清学抗原 。

Objective To isolate proteins with good immunogencity from urea-soluble Schistosoma japonicum(Sj)adult worm antigens and evaluate its potency for schistosomiasis jaonica diagnosis. Methods Urea-soluble adult worm antigens from Sj were analysed by SDS-PAGE and immunoblotting and a 42 kD protein with good immunogencity was fractionated by electrophoretic chromatography. The fractionated 42 kD protein was used to detect IgG of schistosomiasis patients by ELISA. Results A 42 kD protein which reacted to rabbi...