癌胚抗原、端粒酶联合检测鉴别良恶性腹水的临床价值

The Combined Assay of Carcinoembryonic Antigen and Telomerase Activity for the Differential Diagnosis of Ascite

目的:探讨联合检测癌胚抗原(CEA)及端粒酶活性在良恶性腹水鉴别诊断中的临床价值。方法:收集6 7例诊断明确的新鲜腹水标本。ELISA方法检测腹水中CEA含量,用PCR -TRAP技术检测端粒酶活性。结果:恶性组CEA ,端粒酶阳性明显高于良性组,其敏感性分别为5 6 .7%、73.0 % ,特异性分别为83.3%、86 .7%。两者联合检测的敏感性为94 .6 % ,特异性为76 .7% ,准确性为86 .6 %。结论:联合检测CEA以及端粒酶活性可提高良恶性腹水的诊断率,对鉴别腹水性质有重要的临床应用价值。

Objective To evaluate the clinical significance of the assay combin ed carcinoembryonic antigen (CEA) with telomerase activity for the differential diagnosis of malignant and non-malignant ascite. Methods Sixty seven fresh speci mens of peritoneal effusions were obtained from 67 patients with benign or malig nant disease.The CEA was detected by enzyme linked immunosorbent assay (ELISA) a nd the activity of telomerase was detected by PCR-TRAP. Results The positive rate of CEA and telomerase in malignan...