弓形虫致密颗粒蛋白GRA8真核重组表达质粒的体外表达分析

In Vitro Expression of Eukaryotic Expression Plasmid Containing Toxoplasma Gondii GRA8

目的:分析弓形虫致密颗粒蛋白GRA8真核表达质粒的体外表达。方法:大量提取纯化重组真核表达质粒pVAX1-GRA8,瞬时转染培养的vero E-6细胞,RT-PCR和免疫印迹法检测GRA8在细胞中的表达。结果:从转染pVAX1-GRA8后的vero E-6细胞的RNA中扩增出GRA8的特异条带,转染细胞存在弓形虫感染血清识别的特异蛋白质条带。结论:真核表达质粒pVAX1-GRA8在vero E6细胞中获得表达。

Objective To analyse the in vitro expression of eukaryotic expression plasmid containing Toxoplasma gondii dense granule protein GRA8 gene.Methods pVAX1-GRA8 was purified and transfected into Africa green monkey kindey vero cell lines,expression of GRA8 in vero cell lines were detected by RT-PCR and immunoblot with rabbits sera infected with Toxoplasma gondii.Results The gene fragment encoding GRA8 was amplified by RT-PCR from transfected vero E-6 cells;Specific protein expressed in vero cells transfected w...