摘要
目的:建立中药材有效成份氨基酸柱前衍生HPLC分析测定方法。方法:从常见中药材党参和山楂中提取氨基酸,邻苯二甲醛(OPA)柱前衍生,反相高效液相色谱分离测定,以保留时间定性,面积外标法定量。结果:水解温度108℃,提取24 h,可有效提取药材中的氨基酸;梯度洗脱程序为(t,C%):(t:保留时间C:甲醇):(0,0);(9,30);(16,45);(25,60);(28,65);(32,80),二种药材中的十六种氨基酸得到较好分离和定性定量结果,其中天冬氨酸和胱氨酸的平均加样回收率分别为101.0%和94.4%。结论:采用HPLC柱前衍生分离分析党参和山楂中十六种氨基酸的方法灵敏、简便、快捷、高效,适于推广应用,可为解决中药材氨基酸分离测定难题提供参考。
Objective To establish an effective method by which we can determine the content and concentration of amino acids in them.Methods The most suitable procedures are selected and applied to the extraction and further purification of amino acids from common herbs;Employing OPA as a pre-column derivative reagent,reversed-phase(RP) high performance liquid chromatogram(HPLC) is applied to detect.Both qualitative and quantitative analyses are separa-tely performed on sample amino acids recording to retentive time a...
出处
《湖南师范大学学报（医学版）》
2006年第3期34-38,45,共6页
Journal of Hunan Normal University(Medical Sciences)
基金
湖南省中医药管理局科研资助项目(204113)
关键词
党参
山楂
氨基酸
高效液相色谱(HPLC)
柱前衍生
Codonpsispilosulae
hawthorn
amino acids
high performance liquid chromatogram（HPLC）
pre-column derivative reagent
