哮喘家系中的BRS-3编码基因的变异和多态性研究

Sequence Analysis of Mutation or Single Nucleotide Polymorphism(SNP) of BRS-3 in Asthma Patients

目的:观察哮喘家系患者外周血白细胞DNA中蛙皮素受体亚型3(BRS-3)的多态性或基因变异,以了解BRS-3与气道高反应疾病遗传性之间的联系,补充和丰富对气道高反应机制的认识。方法:抽取哮喘患者及正常人外周血白细胞DNA,PCR法扩增BRS-3外显子1和外显子3,测序及进行序列分析。结果:在哮喘患者的BRS-3Exon1和Exon3不存在突变或SNP。结论:推测BRS-3的改变有可能不在编码区,但不排除调控区的改变。

Objective To understand the role of BRS-3 in AHR,the mutation or polymorphism in susceptible genealogy of asthma,which is characterized by AHR,was examined to find relationship between BRS-3 and AHR genetic diseases.Methods DNAs were isolated from peripheral blood leucocytes of asthma patients and normal individuals and the segment of exon1 and exon3 in BRS-3 gene were obtained using PCR for sequence analysis.Results No mutation or SNP loci in both BRS-3 exon1 and exon3 have been found in all sequenced samp...