补肾通络方治疗大鼠C-BSA膜性肾炎的药效学研究

A Pharmacodynamic Study of Bushentongluofang,a Traditional Chinese Medicine formula,in Rats with Experimental Membranous Glomerulonephritis

目的:观察补肾通络方对大鼠膜性肾炎的治疗效果,并探讨其作用机制。方法:大鼠随机分为给药组、模型对照组和正常对照组,阳离子化牛血清白蛋白(C-BSA)免疫法造模,补肾通络方煎剂灌胃给药。测定24 h尿蛋白、血清蛋白、血脂和肾功能等生化指标,用光镜、免疫荧光和电镜技术进行肾组织病理检查。结果:模型组大鼠尿蛋白、血清Ur、Cr、TG、CHO显著高于正常组,而血清TP、Alb相反;肾小球基底膜增厚,上皮侧有免疫复合物沉积,足突广泛融合。与模型组相比,给药组大鼠尿蛋白、血清Ur、Cr、TG、CHO降低,血清TP、Alb升高,肾小球基底膜增厚有所缓解,上皮下免疫沉积物减少,肾组织的其它损伤减轻。结论:补肾通络方能促进膜性肾炎大鼠肾组织病理损伤的修复和肾功能的改善,提示该药方对膜性肾病有较好的疗效。

Purpose To observe the therapeutic effects of Bushentongluofang(BSTLF),a traditional Chinese medicine formula,on the membranous glomerulonephritis(MGN) induced by cationized bovine serum albumin(C-BSA) in rats,and to explore its mechanism of action.Methods Rats were randomly divided into three groups: BSTLF-treated group,the vehicle control group and the normal control group.MGN induced by C-BSA in the two formers.The apozem of BSTLF was intragastrically administered to the rats in treated group for 4 weeks...