摘要
目的:建立同时测量六味补血颗粒中主药有效成分芍药苷和阿魏酸含量的方法。方法:采用Symmetry C18色谱柱(4.6×250 mm,5μm);流动相采用甲醇-0.2%磷酸溶液梯度洗脱;流速1.0 mL/min;检测波长为285 nm;柱温:25℃。结果:芍药苷在17.44~174.4μg.mL-1的范围内峰面积积分值与浓度呈良好的线性关系(r=0.9997),平均回收率为98.9%,RSD=1.29%(n=6);阿魏酸在0.356~14.24μg.mL-1的范围内峰面积积分值与浓度呈良好的线性关系(r=0.9999),平均回收率为98.68%,RSD=2.4%(n=6)。结论:该法准确、简便,重现性好,可作为六味补血颗粒制剂质量控制的有效方法。
Objective to establish an HPLC method for determination of Paeoniflorin and ferulic acid in Liuweibuxue granules.Methods The analytical column was Symmetry C18(4.6×250 mm,5 μm) filled with at 5 μm stationary phase.Methanol-0.2% phosphoric acid gradient elution as mobile phase.The flow rate was 1.0 mL·min-1,the detection wavelength was 285 nm,and the column temperature was 25 ℃.Results The linear range for Paeoniflorin and ferulic acid were 17.44~174.4 μg·mL-1(r=0.9997) and 0.356~14.24 μg·mL-1(r=0.9999),the ...
出处
《湖南师范大学学报（医学版）》
2008年第1期58-60,共3页
Journal of Hunan Normal University(Medical Sciences)
关键词
六味补血颗粒
芍药苷
阿魏酸
含量测定
liuweibuxue granules
ferulic acid
paeoniflorin
determination
