ADFMCHR激活PPARγ抑制人肝癌SMMC-7721细胞生长被引量：4

Growth inhibition of Human Liver Cancer SMMC-7721 Cells by 5-ally-7-gen-difluormethylenechrysin Via Activation of PPARγ

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摘要目的:研究5-烯丙基-7-二氟亚甲基白杨素(ADFMChR)激活过氧化物酶活化因子受体γ(PPARγ)抑制体外培养人肝癌SMMC-7721细胞增殖作用。方法:体外培养人肝癌SMMC-7721细胞。台盼蓝拒染法检测细胞生长;平皿克隆形成法检测细胞锚定依赖性生长;软琼脂克隆形成法检测细胞集落形成能力。结果:细胞计数结果显示,ADFMChR延长SMMC-7721细胞倍增时间,抑制SMMC-7721细胞生长,呈剂量依赖性。但是对预先用PPARγ阻断剂GW 9662孵育30分钟的SMMC-7721细胞,ADFMChR的生长抑制作用明显降低;平皿克隆、软琼脂克隆形成法结果显示,ADFMChR抑制SMMC-7721细胞的锚定依赖性生长能力和锚定非依赖性生长能力,而GW 9662可以降低ADFMChR对SMMC-7721细胞的增值抑制作用。结论:ADFMChR具有抑制人肝癌SMMC-7721细胞生长作用,其机制与激活PPARγ有关。 Objective To investigate the inhibitory effect of 5-ally-7-gen-difluormethylenechrysin on the growth of human liver cancer SMMC-7721 cells by activating PPARγ in vitro.Methods SMMC-7721 cells was cultured in vitro.The growth of SMMC-7721 cells was determined by trypan blue exclusion methods.Plate clone was used to test the anchoring-dependant capacity of SMMC-7721 cells.Soft agar foci formation assay was used to measured the anchoring-independent capacity of SMMC-7721 cells.Results Cell counting results sug...

作者张亮曹建国田英全细云周秀田

机构地区南华大学肿瘤研究所

出处《湖南师范大学学报（医学版）》 2008年第1期20-23,共4页 Journal of Hunan Normal University(Medical Sciences)

关键词肝癌白杨素 5-烯丙基-7-二氟亚甲基白杨素过氧化物酶活化因子受体γ liver cancer chrysin 5-ally-7-gen-difluormethylenechrysin PPARγ

分类号 R735.7 [医药卫生—肿瘤]

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