摘要
目的:优化百合多糖提取、纯化工艺。方法:以水提醇沉法提取百合多糖,savage法去蛋白纯化。在单因素实验的基础上,采用正交试验的方法对影响百合多糖提取、纯化的因素进行优化。结果:百合多糖的最佳提取工艺为:在65℃下,用15倍药材量的蒸馏水提取3次,每次4h。最佳纯化工艺为:样品:氯仿(v∶v)为3∶2,氯仿:正丁醇为2∶1,振摇时间为15min。结论:优化的百合多糖提取纯化工艺简单、稳定,易于产业化规模生产。
Objective The extraction and purification technological condition of lily polysaccharides were optimized.Methods Using the water extraction ethanol precipitation method extract lily polysaccharides,savage method removing proteins.On the basis of single-factor experiments,the extraction and purification teacnological conditions of polysaccharides from lily were optimized by vertical-method.Results The extraction technical of lily polysaccharides is selected as following :15 times of distilled water as the so...
出处
《湖南师范大学学报（医学版）》
2008年第3期7-9,12,共4页
Journal of Hunan Normal University(Medical Sciences)
基金
湖南省科技厅资助科研项目(06FJ3182)
湖南省教育厅资助科研项目(06C497)
关键词
百合多糖
提取
纯化
正交实验
lily polysaccharides
extraction
purification
vertical-method
