摘要
目的:观察8-硝基白杨素(NOChR)诱导人结肠癌HT-29细胞凋亡作用。方法:MTT比色法测定细胞增殖活性;流式细胞分析术检测细胞凋亡率;琼脂糖凝胶电泳观察DNA梯形条带。结果:NOChR抑制HT-29细胞活性,呈浓度依赖性,其IC50值为1.78μM;NOChR具有诱导HT-29细胞凋亡作用;PPARγ特异性阻断剂GW 9662能部分拮抗NOChR诱导HT-29细胞凋亡。结论:NOChR诱导HT-29细胞凋亡作用与其活化PPARγ相关。
Objective To investigate induction of apoptosis of human colon cancer HT-29 cells by 5,7-dihydrox-8-nitrochrysin in vitro.Methods The proliferative activity of HT-29 cells treated with 5,7-dihydrox-8-nitrochrysin was measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-dipheny tertrazolium blue(MTT) colorimetric assay.HT-29 cell apoptotic percentage was detected by flow cytometry with PI staining.DNA ladder bands were observed by DNA agarose gel electrophoresis.Results MTT assay suggested that 5,7-dihydrox-8-nit...
出处
《湖南师范大学学报（医学版）》
2008年第3期13-15,共3页
Journal of Hunan Normal University(Medical Sciences)
基金
湖南省自然科学基金项目(03JJY5009)
湖南省卫生厅医药科研项目(B2006-141C2005-036)
关键词
结肠癌
白杨素
白杨素衍生物
凋亡
human colon cancer
chrysin
chrysin derivatives
apoptosis
