摘要
本文研究了DEAE-Cellulose阴离子交换树脂纯化pCR-HBc-X6-βhCG CTP37质粒DNA的方法。经研究发现纯化的质粒不含RNA和蛋白质污染;其OD260/OD280高于1.8;每2500ml发酵液可得到4.95mg的质粒DNA。
The pCR-HBc-X6-βhCG CTP37 plasmid DNA was purified through DEAE-Cellulose column chromatography.The purified plasmid DNA did not contaminate with RNA and protein.Its OD_(260)/ OD_(280) was higher than 1.8.4.95mg of plasmid DNA per 2500ml culture could be obtained.
出处
《南京晓庄学院学报》
2005年第5期56-59,共4页
Journal of Nanjing Xiaozhuang University
关键词
质粒
提取
纯化
plasmid
extract
purification
