摘要
In order to study the effects of ubiquitin-proteasome pathway (UPP) on mouse oo- cyte meiosis and cleavage, oocytes undergoing maturation and parthenogenetic activation and 1-cell embryos were treated with lactacystin, a specific inhibitor of proteasome. The results indi- cated that the rate of GVBD was not influenced by the treatment, but polar body extrusion, par- thenogenesis and first cleavage were inhibited. Immunofluorescent staining using anti β-tubulin antibody indicated that the continuous treatment of lactacystin from GV stage disorganized microtubules and spindle assembly. When metaphase stage oocytes were treated with the drug, the already formed spindle structure was not affected , but the oocytes were arrested at meta- phases. The 1-cell embryos were arrested at interphase or metaphase of first mitosis when they were incubated in the drug. Proteasome regulatory subunit PA700 was located in the spindle region, as indicated by immunofluorescence. These results suggest that UPP has effects on the process of oocyte meiosis and early cleavage in many aspects, including normal organization of spindle at prophase and segregation of chromosomes at anaphase for normal meiosis.
In order to study the effects of ubiquitin-proteasome pathway (UPP) on mouse oo- cyte meiosis and cleavage, oocytes undergoing maturation and parthenogenetic activation and 1-cell embryos were treated with lactacystin, a specific inhibitor of proteasome. The results indi- cated that the rate of GVBD was not influenced by the treatment, but polar body extrusion, par- thenogenesis and first cleavage were inhibited. Immunofluorescent staining using anti β-tubulin antibody indicated that the continuous treatment of lactacystin from GV stage disorganized microtubules and spindle assembly. When metaphase stage oocytes were treated with the drug, the already formed spindle structure was not affected , but the oocytes were arrested at meta- phases. The 1-cell embryos were arrested at interphase or metaphase of first mitosis when they were incubated in the drug. Proteasome regulatory subunit PA700 was located in the spindle region, as indicated by immunofluorescence. These results suggest that UPP has effects on the process of oocyte meiosis and early cleavage in many aspects, including normal organization of spindle at prophase and segregation of chromosomes at anaphase for normal meiosis.
作者
TAN Xin 1,3 , PENG An 2 , WANG Yongchao 2 & TANG Zuoqing 3 1. School of Life Sciences and Technology, Beijing Institute of Technology, Beijing 100081, China
2. College of Life Sciences, Beijing Normal University, Beijing 100875, China
3. College of Basic Medicine, Capital University of Medical Sciences, Beijing 100054, China
基金
This work was supported by the National Natu-ral Science Foundation of China(Grant No.30270661)
Beijing Natural Science Foundation(5032004)
the Special Funds for Major State Basic Research Project(973)of China(No.G1999053901).
