摘要
Double-barreled (DB) data have been widely used for the assembly of large ge- nomes. Based on the experience of building the whole-genome working draft of Oryza sativa L. ssp. Indica, we present here the prevailing and improved uses of DB data in the assembly pro- cedure and report on novel applications during the following data-mining processes such as ac- quiring precise insert fragment information of each clone across the genome, and a new kind of low-cost whole-genome microarray. With the increasing number of organisms being sequenced, we believe that DB data will play an important role both in other assembly procedures and in future genomic studies.
Double-barreled (DB) data have been widely used for the assembly of large ge- nomes. Based on the experience of building the whole-genome working draft of Oryza sativa L. ssp. Indica, we present here the prevailing and improved uses of DB data in the assembly pro- cedure and report on novel applications during the following data-mining processes such as ac- quiring precise insert fragment information of each clone across the genome, and a new kind of low-cost whole-genome microarray. With the increasing number of organisms being sequenced, we believe that DB data will play an important role both in other assembly procedures and in future genomic studies.
作者
HAN Yujun 1,2 , NI Peixiang 2 , Lü Hong 2 , YE Jia 3 , HU Jianfei 1 , CHEN Chen 2 , HUANG Xiangang 2 , CONG Lijuan 2 , LI Guangyuan 2 , WANG Jing 1 , GU Xiaocheng 1 , YU Jun 2 & LI Songgang 1,2 1. College of Life Sciences, Peking University, Beijing 100871, China
2. Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China
3. James D. Watson Institute of Genome Sciences, Zhejiang University, Hangzhou 310012, China
