Liquid chromatography-electrochemical detection for studying the effects of tetrahydrobiopterin on monoamine neurotransmitters in rat striatum

Tetrahydrobiopterin(BH4)is an essential co-factor in the biosynthesis of monoamine neurotransmitters.A nano-Pt/Pd modified electrode as the electrochemical detector(ED)for high-performance liquid chromatography(HPLC)coupled with microdialysis sampling,is used to explore the effect of administration of BH4 on the monoamine neurotransmitters in the rat striatum.The researches demonstrate that the contents of dopamine(DA),5-hydroxytryptamine(5-HT),5-hydroxyindoleacetic acid(5-HIAA)and homovanillic acid(HVA)increase significantly with the administration of BH4.The pharmaceutical kinetics is carried out to research into the time course of BH_(4) effect on the concentration of monoamine neurotransmitters in rat striatum,which pro-vides reliable data for pathology and pharmacology research on neuroscience.