抗寒添加剂对大鼠的饲喂效应及安全性评价

The Effect and The Security of Cold-resistant Additive on Rats

将80只Wistar大鼠随机分为4组,设1.0、2.5、5.0g/kg3个剂量组和对照组,进行抗寒添加剂30d喂养试验。结果表明,饲喂添加剂后,各组动物生长发育良好,高剂量组大鼠体重增长较快。第15d检查,各剂量组血液学指标与对照组比较差异不显著;高剂量组有多项血液生化指标与对照组相比差异显著,肝脏器系数小于对照组;其它检查指标均正常。第30d检查,各剂量组大鼠血液中淋巴细胞在白细胞中所占比例高于对照组,但其绝对含量无统计学差异,其它血液学指标与对照组比较无显著性差异;中剂量组有多项血液生化指标与对照组相比差异显著;脏器系数及病理组织学检查未见明显异常。由此可推之:在试验计量范围内应用抗寒食品添加剂,可使机体代谢功能增强,对生长发育无不良影响。部分检测指标改变,与抗寒机制形成有关,而且随用药时间延长有自主恢复的趋势。

A total of 80 Wistar rats were randomly divided into four groups: 3 experiment groups and 1 control. The experiment groups were feed cold-resistant additive for 30d at dose of 1.0,2.5 and 5.0 g/kg. The results showed that growing and developing of rats were normal after feed additive, and increase of the body weight were quickly at high dose group in 10 day. There was no significant difference among the targets of blood in test groups and those of control group at 15 day. Significant difference were observed among many biochemical indexes of blood in high dose group and those of control group. At 30 day, The rate of LYM in WBC among test groups were higher than that of control group. But no statistical significances were got in absolute content. Other blood index were similar to that of control group. Many biochemical indexes of blood in middle dose group were increased. Organic coefficients and pathological histology of live, kidney, spleen, adrenal gland and thyroid gland were normal. Conclusion: When rats were fed cold-resistant additive at larger dose and longer time, some index appear charged, but some of they can gradually return to normal. These charges may be physiological reaction in the course of set up cold-resistant mechanism.