中国对虾5个地理群体的RAPD分析

RAPD analysis of five geographic stocks of Fenneropenaeus chinensis

采用随机扩增多态DNA(RAPD)技术对取自辽东湾、渤海湾、海州湾、乳山湾及海洋岛5个群体的中国对虾共95个个体的遗传多样性进行分析。14个随机引物共检测出103个位点(200～2500bp),各群体的多态位点比例在31.07%～34.95%之间。遗传距离显示中国对虾群体间有一定遗传分化,其中辽东湾和乳山湾群体间的遗传距离最大(0.0742),而辽东湾和渤海湾群体间的遗传距离最小(为0.0243),群体间的遗传分化系数为0.2083。整体来看,中国对虾的遗传变异水平较低,遗传多态度为0.1621。用UPGMA对5个群体进行聚类分析,构建谱系关系图。

RAPD (random amplified polymorphic DNA) was employed Fenneropenaeus chinensis. Samples were collected from Liaodong Bay, Bohai Bay, Haizhou Bay, Rushan Bay and Haiyangdao). Fourteen 10bp RAPD primers were used for DNA amplification and 19 samples of each stock were analysed. Totally 103 markers were detected in a range of 200-2500bp. Percentage of polymorphic loci of each stock was from 31.07% to 34.95% and genetic diversity was from 0.1056 to 0.1192. Genetic distance between stocks was from 0.0243 to 0.0742 and the index of genetic diversity of the stock was 0.2083, which indicated that stocks differentiated from each other genetically. Clustering of the stocks was analysed by UPGMA. The RAPD revealed that the overall genetic diversity of F. chinensis was low.