反相高效液相色谱法测定白苏子和白苏子油中迷迭香酸的含量

Determination of rosmarinic acid in seeds and oils of Perilla frutescens(L.)Britton

采用反相高效液相色谱法测定了白苏子及其油中迷迭香酸的含量。使用KromasilC18(250mm×4.6mm,5μm)色谱柱,流动相为甲醇-0.1%磷酸水溶液(40:60),光电二极管阵列检测器,检测波长331nm,流速0.8mL/min,柱温28℃。迷迭香酸进样量在0.20～4.00μg时呈现良好的线性关系,线性回归方程为Y=3.02×105X-3.50×103,r=0.9999,平均加样回收率为97.3%,RSD为2.1%。白苏子中迷迭香酸的含量为0.2475mg/g,约为白苏子油中迷迭香酸含量0.0794mg/g的3.12倍。白苏子采用压榨法生产白苏子油时,活性成分迷迭香酸大部分残留在油粕中。方法灵敏、准确,重现性好,适用于白苏子和白苏子油中迷迭香酸的定量检测分析。

The contents of rosmarinic acid(RA)in seeds and oils of Perilla frutescens(L.)Britton were determined by RP-HPLC.Rosmarinic acid was separated and analyzed on Kromasil C18 column(250mm×4.6mm,5μm)using methanol-phosphoric acid solution(40:60)as mobile phase at flow rate of 0.8mL/min.UV detection wavelength was set at 210nm and the column temperature was 28℃.An average recovery of 97.3% was obtained with a RSD of 2.1% in the range of 0.20～4.00μg for rosmarinic acid.The linear regression equation of dyclonine hydrochloride was Y=3.02×105X-3.50×103(r=0.9999)for HPLC method.The contents of rosmarinic acid in seeds and oils were 0.2475mg/g and 0.0794mg/g.The content of rosmarinic acid in seeds was 3.12times higher than that of in oils.Active Component of rosmarinic acid remained mostly in oil meal by mechanical extrusion.The method was sensitive and accurate.It has good repeatability and a proper method for determination of rosmarinic acid in seeds and oils of Perilla frutescens(L.)Britton.