温清饮提取物对应激性溃疡大鼠血清TNF-α、NO含量的影响

EFFECTS OF WENGQINGYIN EXTRACT ON SERUM TNF-α AND NO IN RATS WITH STRESS ULCER

目的:观察温清饮(WQY)抗应激性溃疡的作用机制。方法:采用束缚-水浸应激溃疡大鼠模型。观察温清饮大、中、小3个剂量对大鼠应激性溃疡发生情况的影响,并应用ELISA法检测各组大鼠血清TNF-α水平,紫外分光光度法测定血清NO含量。结果:WQY可显著升高应激性溃疡大鼠NO含量,降低血清TNF-α水平。结论:降低TNF-α水平,增加NO含量可能是WQY抗溃疡作用机制之一。

Objective:To discuss the mechanism of wenqingyin on stress ulcer.Methods: Models were made by fetter-water immersion.Rats were divided into 6 groups,normal,model,western medicine control and wenqingyin three dose groups,every group was given corresponding medicines,the changes of serum TNF-α and NO in each group were determined by ELISA and ultraviolet spectrophotometer.Results: The TNF-α level in the model group was obviously higher than that of normal,which was lowered significantly in the western medicine and wenqingyin three dose groups .The NO content in model group was lowered obviously in model group,which was significantly elevated in each treated group,there were obvious difference between each treated group and model group.Conclusion: The ulcer-preventive effects of wenqingyin maybe ralated with lowering TNF-α and elevating NO content.