Bcl-2和Cox-2在子宫内膜癌中的表达

Expressions of Cox-2 and Bcl-2 in endometrial carcinoma tissue

目的:研究Bcl-2和Cox-2在子宫内膜癌中的表达,并对其临床意义做初步探讨。方法:采用免疫组织化学SP法检测25例正常子宫内膜,15例不典型增生子宫内膜,56例子宫内膜癌Bcl-2和Cox-2的分布及表达。结果:(1)Cox-2在正常子宫内膜、子宫内膜不典型增生、子宫内膜癌中的阳性表达率逐渐增强,阳性率分别为12%,40%及69.64%,差异高度显著(P<0.05),Cox-2的表达强度与组织分化程度、肌层浸润深度正相关(P<0.05)。(2)Bcl-2在增生期子宫内膜、子宫内膜不典型增生、子宫内膜腺癌中的表达强度逐渐降低,阳性率分别为96%,73.33%,44.64%,差异显著(P<0.05)。内膜腺癌中,Bcl-2的表达强度与组织分化程度、肌层浸润深度、淋巴结转移负相关(均P<0.05)。(3)内膜腺癌中,Bcl-2与Cox-2的表达明显相关性(P<0.05)。结论:Cox-2及Bcl-2可能与子宫内膜癌的早期形成有一定关系,共同参与子宫内膜腺癌的发生、发展。

Objective:To investigate the expression of Cox-2 and Bcl-2 genes and clinical significance in endometrial adenocarcinoma.Methods:A cross-sectional study was conducted in 96 women in the following categories:(1)normal endometrium(n=25),(2) atypical hyperplastic endometrium(n=15),and(3)endometrial carcinoma(n=56).The expression of Cox-2 and Bcl-2 was investigated by SP immunohi-immunohistochemical assay.Results:(1) The expression of Cox-2 increased progressively from normal and atypical hyperplastic endometrium to carcinoma(12%,40% and69.64%,respectively) with a significant difference(P<0.05).The expression of Cox-2 in endometrial adenocarcinoma had a great relevancy with the histologicalgrade and myometrial invasion.(2)The expressioof Bcl-2 decreased gradually from normal and atypical hyperplasticendometrium to carcinoma with positivity of 96%, 73.33% and 44.64%,respectively,with a significant difference(P<0.05).The expression of Bcl-2 in endometrial adenocarcinoma was reversely related with histologicalgrade,lymph node metastasis and myometrial invasion(P<0.05;P<0.05;P<0.05).(3) A noticeable relation was found between Bcl-2 gene expression and Cox-2 gene expression.Conclusion:The decreased expression of Bcl2 and Cox-2 might be one of early events in the occurrence of endometrial carcinoma.The above two factors were highly expressed in endometrial adenocarcinoma and closely related with the clinical-pathological parameters.