清肠汤对大肠湿热型溃疡性结肠炎大鼠组织NF-κBp65及TLR4的影响

Influences of Qingchang Decoction on tissue NF-κB p65 and TLR4 of Rats with Ulcerative Colitis of Large Intestine Damp-heat Syndrome Type

目的观察清肠汤对大肠湿热型UC大鼠肠组织NF-κB p65及TLR4的表达的影响。方法采用葡聚糖硫酸钠加乙酸配合饮食加环境复合法,复制大肠湿热型UC大鼠模型。采用免疫组化染色法检测各组大鼠结肠组织NF-κB p65和TLR4的表达。结果与正常组相比,模型组大鼠结肠组织中NF-κB p65、TLR4的表达显著增高(P<0.01),清肠汤各剂量组和SASP组NF-κB p65、TLR4的表达较模型组明显降低(P<0.01)。结论NF-κB p65、TLR4表达降低。很可能是清肠汤治疗大肠湿热型UC大鼠的机理。

Objective To observe the influences of Qingchang Decoction on tissue NF-κB p65 and TLR4 of Rats with Ulcerative Colitis of Large Intestine Damp-heat Syndrome Type.Methods Large intestine damp-heat type UC was duplicated on rats by composite approach of dextran sultate sodium plus acetic acid combined with diet and environment.Using immunohistochemical staining for detecting NF-κB p65,TLR4 expression in rat colon tissue.Results Compared with normal group,model group colon tissue NF-κB p65,TLR4 expression was significantly higher(P<0.01) after treatment,and Qingchang decoction groups(all dosages) and SASP group were lower than that in model group significantly(P <0.01).Conclusion The decreased expressions of NF-κB p65 and TLR4 may be the mechanism of Qingchang decoction treating large intestine damp-heat syndromes type UC.